• Acquiring an enriched population of a given cell type using

  2018-10-20

  

  Acquiring an enriched population of a given cell type using a fluorescence-based reporter line has many advantages but requires that the reporter expression is specific to the cell type to be enriched. Glial fibrillary acidic protein (GFAP) is a well-known marker for astrocytes; its expression is dy

• Inhibition of endogenous Runx activity with

  2018-10-20

  

  Inhibition of endogenous Runx1 activity with Ro5-3335 led to a decrease in neurosphere size as well as the percentage of cells incorporating EdU (Fig. 2). Thus, inhibition of Runx1 results in decreased NSPC proliferation, suggesting that endogenous Runx1 protein expressed in neurosphere cultures is

• apexbio calculator Mammalian muscle differentiation is gover

  2018-10-20

  

  Mammalian muscle differentiation is governed by the expression of a series of master regulatory transcription factors, collectively called myogenic regulatory factors (MRFs) (Bentzinger et al., 2012; Molkentin and Olson, 1996). Proliferating myoblasts start expressing MRFs, i.e. MyoD, myogenin, MRF4

• Background green fluorescence was detected in the lentiviral

  2018-10-20

  

  Background green fluorescence was detected in the lentiviral reprogrammed MML-6838-CL2 iPSC line and a control episomal (non-GFP) reprogrammed iPSC, indicating silencing (Fig. 1B). Pluripotency was confirmed by detecting expression of SOX2, OCT4, SSEA4, TRA1-61 and TRA1-80 pluripotency-associated ma

• br Results br Discussion Here we

  2018-10-20

  

  Results Discussion Here, we demonstrate that the status of the X chromosome is dynamic during human female somatic cell reprogramming. Extending previous studies that examined X chromosome status in iPSCs after completion of reprogramming, we determined the change of X chromosome status in 5-a

• Functional human endothelial cells differentiated

  2018-10-20

  

  Functional human endothelial cells differentiated from hPSCs could be beneficial for many potential clinical applications (Burridge et al., 2012; Kaupisch et al., 2012; Levenberg et al., 2002; van der Meer et al., 2013), including engineering new blood vessels, endothelial cell transplantation into

• Taken together we here show

  2018-10-20

  

  Taken together, we here show that the recently identified marker SPOT14 labels low-proliferating NSPCs in the hippocampus that react dynamically to positive and negative neurogenic stimuli in vivo. These data further validate the usefulness of this restrictive and functionally relevant marker to ana

• br Results and Discussion Using the neurosphere protocol Pic

  2018-10-20

  

  Results and Discussion Using the neurosphere protocol (Piccirillo et al., 2009), we established stable patient-derived cell cultures from dissociated tumor tissue from 12 cases of primary GBM (Table S1 available online). In order to exclude the possibility of in vitro aberrations and culture sele

• However before application in a clinical context

  2018-10-20

  

  However before application in a clinical context, a number of issues still need to be considered. Indeed, SSC transplantation in cancer patients requires techniques to exclude cancer cell contamination, because implantation of as few as 20 leukemic ck2 inhibitor can result in cancer relapse (Jahnuka

• melanocortin 1 receptor br Experimental Procedures br Acknow

  2018-10-20

  

  Experimental Procedures Acknowledgments Introduction Germ melanocortin 1 receptor are the only cells that continue to be reprogrammed throughout their lifetime in order to transfer genetic information to subsequent generations (Sasaki and Matsui, 2008). Germ cells have unique characteristi

• Several possibilities could explain the aberrant expression

  2018-10-20

  

  Several possibilities could explain the aberrant expression of ATM in Q3SC. Exon skipping has been observed in several genes (Dutertre et al., 2010; Nevo et al., 2012) and is believed to be influenced by ATM function (Tresini et al., 2015). By amplifying cDNA with PCR primers targeting sites outside

• For live cell imaging we

  2018-10-20

  

  For live-cell imaging, we established a 96-well plate-based screening assay to assess expression of the pluripotency-associated surface marker TRA-1-60, a more reliable and specific marker for predicting successful reprogramming and iPS cell derivation than other markers including AP (Mali et al., 2

• EWS FLI a widely recognized fusion oncogene for Ewing

  2018-10-20

  

  EWS-FLI1, a widely recognized fusion oncogene for Ewing sarcomas, is generated by the chromosomal translocation of t(11;22) (q24;q12), which consists of the N-terminal transactivator domain of the EWS gene and the C-terminal ETS DNA binding domain of the FLI1 gene. The resulting EWS-FLI1 fusion prot

• acetylcholine receptors MYC is not a pluripotency

  2018-10-20

  

  MYC is not a pluripotency factor, but it substantially improves reprogramming dynamics, primarily because MYC accelerates cell proliferation (Sabo et al., 2014; Walz et al., 2014), providing an opportunity for the somatic genome to gradually reshape its epigenetic landscape. MYC is critical at an ea

• Telomeres are repetitive DNA structures at the

  2018-10-20

  

  Telomeres are repetitive DNA structures at the chromosome ends that safeguard them from DNA repair and degradation activities. This protective function requires a proper telomere length and the binding of a six-protein complex known as shelterin, including TRF1, which is essential for telomere prote

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